TISSUE PROCESSING~NEW TOPIC

Definition : Any treatment of tissue necessary to impregnate them with a solid medium to
falicitate the production of sections for microscopy.


Aims : a) to embed the tissue in a solid medium

b) to enable the knife to cut the section with little damage to knife or tissue


4 major steps :

1. Dehydration

2. Clearing

3. Infiltration and Impregnation

4. Embedding


PROCEDURE

50% Alcohol - 1 ½ hours

70% Alcohol - 1 ½ hours

80% Alcohol - 1 ½ hours * can proceed to overnight at this step

95% Alcohol - 1 ½ hours

100% Alcohol I - 1 ½ hours

100% Alcohol II - 1 ½ hours

100% Alcohol III - 1 ½ hours

Equal Alcohol and Toluene - 1/2 hours

Toluene - Overnight

Paraffin I - 2 hours

Paraffin II - 2 hours

Paraffin III - 2 hours